| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Freimanis, Alyssa K (14)
|
GUSHAC |
5:37.52 |
5:30.40 |
-7.12 |
| 2 |
Givens, Leah M (14)
|
GUSHRK |
5:36.38 |
5:34.77 |
-1.61 |
| 3 |
Becker, Peyton R (14)
|
GUSHRK |
5:58.28 |
5:34.90 |
-23.38 |
| 4 |
Vidinha, Alexandra J (14)
|
GUSHRK |
5:59.56 |
5:36.24 |
-23.32 |
| 5 |
Linden, Alexandra E (14)
|
GUSHRK |
5:49.35 |
5:45.99 |
-3.36 |
| 6 |
Hilbrich, Alexandria L (13)
|
GUSCAT |
|
5:50.88 |
- |
| 7 |
Bentley, Elaine E (14)
|
GUSHRK |
5:58.72 |
5:52.70 |
-6.02 |
| 8 |
Edwards, Isabel A (14)
|
GUSCAT |
5:54.14 |
5:58.60 |
4.46 |
| 9 |
Hewitt, Victoria N (13)
|
GUTEST |
|
6:07.51 |
- |
| 10 |
Vestal, Emma L (13)
|
GUSHRK |
6:17.45 |
6:20.71 |
3.26 |
| 11 |
Merry, Sophia L (14)
|
GUSPA |
|
6:22.89 |
- |
| 12 |
Diersen, Alyssa Y (14)
|
GUSWAT |
6:33.18 |
6:23.55 |
-9.63 |
| 13 |
Broughton, Mya A (14)
|
GUHCAP |
|
6:41.89 |
- |
| 14 |
Roosa, Catherine E (14)
|
GUSPA |
|
6:59.37 |
- |